Part:BBa_K1129018:Design

CaDXMT1 under pTET constitutive promoter

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1057
  Illegal BglII site found at 1153
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

We have yet to test for expression, and we may find that codon optimization is needed.

Source

We used part BBa_J23118, a constitutive promoter, from the 2012 Distribution Kit and BBa_K801072 from the 2013 Kit. The latter was submitted by the TU Munich 2012 team.